7EX0

Crystal structure of Ebinur Lake virus cap snatching endonuclease (K108A mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	69.808, 41.452, 85.766
Unit cell angles	90.00, 104.72, 90.00

Refinement procedure

Resolution	30.210 - 2.070
R-factor	0.2042
Rwork	0.203
R-free	0.23020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xi5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.070	4.460	2.070
Rmerge	0.040	0.021	0.455
Rmeas	0.047	0.025	0.533
Rpim	0.024	0.013	0.273
Total number of observations	104989
Number of reflections	29345	3037	2861
<I/σ(I)>	15.3
Completeness [%]	99.8	98.9	99.7
Redundancy	3.6	3.4	3.6
CC(1/2)		0.999	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.1	289	PEG 3350