7EWY

Crystal structure of Ebinur Lake virus cap snatching endonuclease (D79A mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	41.171, 69.670, 72.751
Unit cell angles	90.09, 89.98, 90.02

Refinement procedure

Resolution	25.390 - 2.010
R-factor	0.2068
Rwork	0.205
R-free	0.24260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xi5
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	69.640	69.640	2.060
High resolution limit [Å]	2.010	8.990	2.010
Rmerge	0.111	0.076	0.799
Number of reflections	52745	587	3879
<I/σ(I)>	6.9
Completeness [%]	98.0	98.5	96.8
Redundancy	3.6	3.5	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.9	289	PEG 3350