7EWV

Crystal structure of Ebinur Lake virus cap snatching endonuclease (H34A mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	70.754, 41.009, 75.018
Unit cell angles	90.00, 99.81, 90.00

Refinement procedure

Resolution	29.640 - 2.610
R-factor	0.2266
Rwork	0.226
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xi5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.700
High resolution limit [Å]	2.610	5.620	2.610
Rmerge	0.086	0.054	0.437
Rmeas	0.099	0.062	0.508
Rpim	0.047	0.030	0.251
Total number of observations	39969
Number of reflections	11695	1137	1178
<I/σ(I)>	11.2
Completeness [%]	88.2	80.9	89.1
Redundancy	3.4	3.4	3.3
CC(1/2)		0.998	0.795

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.1	289	PEG 3350