7EWU

Crystal structure of Ebinur Lake virus cap snatching endonuclease (WT)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	70.812, 41.587, 145.313
Unit cell angles	90.00, 101.58, 90.00

Refinement procedure

Resolution	35.950 - 2.110
R-factor	0.2422
Rwork	0.240
R-free	0.28510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xi5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.11.1_2575)
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.190
High resolution limit [Å]	2.110	4.540	2.110
Rmerge	0.085	0.058	0.566
Rmeas	0.096	0.066	0.636
Rpim	0.043	0.030	0.286
Total number of observations	210591
Number of reflections	47354	4854	4758
<I/σ(I)>	10
Completeness [%]	97.9	96.4	99.2
Redundancy	4.4	4.1	4.6
CC(1/2)		0.997	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.7	289	PEG 3350