7EWO

Crystal Structure of D67A, E68P double mutant of O-acetyl-L-serine sulfhydrylase from Haemophilus influenzae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-09-26
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5414
Spacegroup name	I 41
Unit cell lengths	113.212, 113.212, 43.475
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.590 - 2.400
R-factor	0.1947
Rwork	0.192
R-free	0.25660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ho1
RMSD bond length	0.007
RMSD bond angle	0.905
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.590	2.483
High resolution limit [Å]	2.397	2.397
Rmerge	0.200	0.230
Number of reflections	10586	786
<I/σ(I)>	31.8	2.8
Completeness [%]	95.9	73.94
Redundancy	5.6	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M HEPES, 1.3M Sodium Citrate