7EWJ
Toxin-antitoxin complex from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9000 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 164.509, 164.509, 232.209 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.770 - 2.000 |
| R-factor | 0.1883 |
| Rwork | 0.188 |
| R-free | 0.21880 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.649 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 205519 | 32910 |
| <I/σ(I)> | 19.5 | |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 7.12 | 7.17 |
| CC(1/2) | 1.000 | 0.789 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.0 M ammonium sulfate, 100 mM CAPS/Sodium hydroxide, pH 10.5, 200 mM lithium sulfate |
