7EWF

Selenomethionine-substituted structure of S. cerevisiae Csn12 in complex with Thp3 and Sem1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97852
Spacegroup name	P 32 2 1
Unit cell lengths	116.319, 116.319, 127.347
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.580 - 2.850
R-factor	0.2142
Rwork	0.212
R-free	0.25670
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.823
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CRANK2
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.850	2.850
Rmerge	0.147	0.895
Number of reflections	23736	1168
<I/σ(I)>	23.44	2.62
Completeness [%]	100.0	97.3
Redundancy	16.6
CC(1/2)	1.000	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	12% PEG3350, 100 mM Sodium malonate buffer (pH 5.0) and 3% Methanol