7EW5

immune complex of HPV6 L1 pentamer and neutralizing antibody 13H5

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	123.15
Detector technology	PIXEL
Collection date	2013-10-20
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	139.090, 208.309, 205.948
Unit cell angles	90.00, 95.61, 90.00

Refinement procedure

Resolution	49.019 - 3.606
R-factor	0.2542
Rwork	0.252
R-free	0.29300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1plg 5y9e
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.670
High resolution limit [Å]	3.600	3.610
Number of reflections	133760	12913
<I/σ(I)>	5.1
Completeness [%]	99.9	99.9
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293.15	0.1M Hepes pH 7.0, 8.2% PEG 20000