7EV7
Bovine heart cytochrome c oxidase in the carbon monoxide-bound fully reduced state at a 50 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 50 |
| Detector technology | CCD |
| Collection date | 2017-06-30 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 181.539, 203.655, 177.569 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.743 - 1.700 |
| R-factor | 0.1595 |
| Rwork | 0.159 |
| R-free | 0.17830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b1b |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.617 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((1.13-2998-000)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 1.610 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.102 | 1.396 |
| Rpim | 0.033 | 0.565 |
| Number of reflections | 8129618 | 21052 |
| <I/σ(I)> | 38.18 | 2.16 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 9.5 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.8 | 277 | PEG 4000, Sodium phosphate buffer |
