7EUL
Crystal structure of C86H-H196S mutant of N(omega)-hydroxy-L-arginine hydrolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.499, 50.343, 52.941 |
Unit cell angles | 90.00, 99.42, 90.00 |
Refinement procedure
Resolution | 41.930 - 1.450 |
R-factor | 0.1702 |
Rwork | 0.169 |
R-free | 0.19330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6luh |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.930 | 41.930 | 1.470 |
High resolution limit [Å] | 1.450 | 7.940 | 1.450 |
Rmerge | 0.062 | 0.052 | 0.381 |
Rmeas | 0.070 | 0.060 | 0.430 |
Rpim | 0.032 | 0.028 | 0.195 |
Total number of observations | 181105 | 1147 | 9071 |
Number of reflections | 39175 | 267 | 1932 |
<I/σ(I)> | 13 | 25.2 | 3.1 |
Completeness [%] | 99.9 | 99.4 | 99.8 |
Redundancy | 4.6 | 4.3 | 4.7 |
CC(1/2) | 0.996 | 0.989 | 0.908 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 298 | PEG 4000, MgCl2 |