7ETS
Crystal structure of crp protein from Gardnerella Vaginalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.953, 85.471, 56.022 |
| Unit cell angles | 90.00, 116.69, 90.00 |
Refinement procedure
| Resolution | 28.000 - 2.220 |
| R-factor | 0.2402 |
| Rwork | 0.238 |
| R-free | 0.27590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4byy |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.555 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.302 |
| High resolution limit [Å] | 2.220 | 2.223 |
| Number of reflections | 24348 | 2226 |
| <I/σ(I)> | 2.318 | |
| Completeness [%] | 98.3 | |
| Redundancy | 3.2 | |
| CC(1/2) | 0.996 | 0.947 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 5% 2-propanol, 100mM Imidazole/Hydrochric acid, pH 7.5 |
