7ET6

Crystal structure of Arabidopsis TEM1 B3-DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 43 2 2
Unit cell lengths	68.760, 68.760, 162.987
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.231 - 2.700
R-factor	0.2338
Rwork	0.232
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ldw
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.065	0.988
Rmeas	0.072
Rpim	0.030	0.446
Number of reflections	12666	1227
<I/σ(I)>	18.6	1.2
Completeness [%]	99.2
Redundancy	5.6
CC(1/2)		0.513

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.1 M MgAc, 0.1 M MES pH 6.5, 10% PEG 10000