7ET0
Crystal structure of the complex formed by Wolbachia cytoplasmic incompatibility factors CinA and CinB from wPip
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 |
| Unit cell lengths | 85.805, 87.118, 92.215 |
| Unit cell angles | 111.27, 90.97, 114.67 |
Refinement procedure
| Resolution | 46.328 - 2.200 |
| R-factor | 0.2195 |
| Rwork | 0.219 |
| R-free | 0.24850 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
| High resolution limit [Å] | 2.194 | 5.970 | 2.200 |
| Rmerge | 0.119 | 0.105 | 1.381 |
| Rmeas | 0.126 | 0.112 | 1.499 |
| Rpim | 0.043 | 0.039 | 0.568 |
| Number of reflections | 109042 | 5563 | 5317 |
| <I/σ(I)> | 25.9 | ||
| Completeness [%] | 97.2 | 98.9 | 95 |
| Redundancy | 8.4 | 8.7 | 6.3 |
| CC(1/2) | 0.958 | 0.824 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 289.15 | buffer A (0.2M L-Proline, 0.1M Hepes pH 7.5, 24% w/v polyethylene glycol 1500) : buffer B (0.1M citric acid pH 3.5, 34% v/v polyethylene glycol 200) = 4:1 |
