7ESZ
Crystal structure of the complex formed by Wolbachia cytoplasmic incompatibility factors CinA and CinB with Mn2+ from wPip
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97876 |
| Spacegroup name | P 1 |
| Unit cell lengths | 85.466, 86.380, 93.018 |
| Unit cell angles | 110.06, 91.86, 115.38 |
Refinement procedure
| Resolution | 41.071 - 2.476 |
| R-factor | 0.2006 |
| Rwork | 0.198 |
| R-free | 0.25020 |
| Structure solution method | SAD |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.476 | 6.780 | 2.500 |
| Rmerge | 0.183 | 0.079 | |
| Rmeas | 0.211 | 0.096 | |
| Rpim | 0.144 | 0.055 | |
| Number of reflections | 73644 | 3559 | 3698 |
| <I/σ(I)> | 11.9 | ||
| Completeness [%] | 97.1 | 93.4 | 96.6 |
| Redundancy | 3 | 3 | 2.8 |
| CC(1/2) | 0.983 | 0.106 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 289.15 | buffer A (0.2M L-Proline, 0.1M Hepes pH 7.5, 24% w/v polyethylene glycol 1500): buffer B (0.1M citric acid pH 3.5, 34% v/v polyethylene glycol 200) = 4:1 |
