7ESY

Crystal structure of the complex formed by Wolbachia cytoplasmic incompatibility factors CidA and CidBND1-ND2 from wPip

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97909
Spacegroup name	P 21 21 2
Unit cell lengths	102.361, 154.556, 83.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.587 - 2.297
R-factor	0.2178
Rwork	0.217
R-free	0.24330
Structure solution method	SAD
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.379
High resolution limit [Å]	2.297	2.297
Rmerge	0.112	1.729
Number of reflections	59653	5741
<I/σ(I)>	16.6
Completeness [%]	96.5	87.08
Redundancy	12.8	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		289	0.2M calcium chloride dehydrate, pH 5.1, 20% w/v polyethylene glycol 3350