7ESX
Crystal structure of Wolbachia cytoplasmic incompatibility factor CidA from wPip
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-13 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.671, 63.376, 66.716 |
| Unit cell angles | 82.59, 70.21, 89.37 |
Refinement procedure
| Resolution | 47.470 - 1.800 |
| R-factor | 0.166 |
| Rwork | 0.165 |
| R-free | 0.19800 |
| Structure solution method | SAD |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 0.655 |
| Rmeas | 0.106 | 0.067 |
| Rpim | 0.057 | 0.036 |
| Total number of observations | 267156 | |
| Number of reflections | 76435 | 3846 |
| <I/σ(I)> | 26.4 | |
| Completeness [%] | 95.6 | 95.2 |
| Redundancy | 3.5 | 3.6 |
| CC(1/2) | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 289 | 0.1M Tris pH 8.0, 30% w/v polyethylene glycol monomethyl ether 2000 |
