7ESQ

Structure and mutation analysis of the hexameric P4 from Pseudomonas aeruginosa phage phiYY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	I 21 3
Unit cell lengths	170.260, 170.260, 170.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.130 - 1.850
R-factor	0.1864
Rwork	0.185
R-free	0.20410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4blp
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.130	1.960
High resolution limit [Å]	1.850	1.850
Number of reflections	687024	112408
<I/σ(I)>	26.68
Completeness [%]	99.8
Redundancy	9.8
CC(1/2)	1.000	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	291	200 mM NH4 tartrate, 20% PEG2000 MME, phosphate pH 7.9