7ESO
Structure and mutation analysis of the hexameric P4 from Pseudomonas aeruginosa phage phiYY
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | H 3 |
Unit cell lengths | 241.075, 241.075, 152.950 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.130 - 2.450 |
R-factor | 0.1817 |
Rwork | 0.180 |
R-free | 0.20760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4blp |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.130 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmeas | 0.167 | 0.120 |
Number of reflections | 242159 | 62177 |
<I/σ(I)> | 10.3 | 2.4 |
Completeness [%] | 99.7 | |
Redundancy | 10.2 | |
CC(1/2) | 0.995 | 0.694 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 600 mM K/Na tartrate, 100 mM Tris-HCl pH 8.5 |