7ESJ

Acinetobacter baumannii membrane-bound lytic murein transglycosylase A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-10-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.998803
Spacegroup name	C 1 2 1
Unit cell lengths	97.730, 67.440, 142.370
Unit cell angles	90.00, 98.87, 90.00

Refinement procedure

Resolution	29.266 - 2.060
R-factor	0.2207
Rwork	0.218
R-free	0.26720
Structure solution method	AB INITIO PHASING
Data scaling software	XDS
Phasing software	Arcimboldo
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.270	2.349
High resolution limit [Å]	2.060	2.060
Rmerge	0.069	0.916
Number of reflections	56717	18347
<I/σ(I)>	13.9
Completeness [%]	99.9
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	20%(w/v) PEG-8000, 0.1M CHES pH9.5