7ESI
Crystal structure of the collagenase unit of a Vibrio collagenase from Vibrio harveyi VHJR7 at 1. 8 angstrom resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.444, 50.068, 90.681 |
| Unit cell angles | 90.00, 104.72, 90.00 |
Refinement procedure
| Resolution | 49.790 - 1.800 |
| R-factor | 0.1601 |
| Rwork | 0.159 |
| R-free | 0.18670 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.790 | 1.870 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.051 | 0.138 |
| Rmeas | 0.061 | 0.164 |
| Rpim | 0.033 | 0.087 |
| Number of reflections | 59569 | 5891 |
| <I/σ(I)> | 23.95 | 8.87 |
| Completeness [%] | 98.9 | 98.51 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.995 | 0.972 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M sodium acetate trihydrate, 0.1 M Tris-HCl (pH 8.5), 30% (w/v) polyethylene glycol 4000 |
