7ES1
glycosyltransferase in complex with UDP and ST
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.843, 83.042, 109.167 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.089 - 1.660 |
| Rwork | 0.179 |
| R-free | 0.20430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | apo form of this protein |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.438 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.090 | 46.110 | 1.690 |
| High resolution limit [Å] | 1.660 | 4.500 | 1.660 |
| Rmerge | 0.089 | 0.070 | 1.087 |
| Number of reflections | 55463 | 3020 | 2623 |
| <I/σ(I)> | 9 | 20.9 | |
| Completeness [%] | 99.9 | 100 | 96.8 |
| Redundancy | 5.9 | 5.8 | 3.2 |
| CC(1/2) | 0.996 | 0.992 | 0.499 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 4000 and 0.1 M HEPES pH 6.5 |
