7ERX
Glycosyltransferase in complex with UDP and STB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.482, 83.173, 109.391 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.297 - 1.920 |
Rwork | 0.189 |
R-free | 0.21350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ery |
RMSD bond length | 0.008 |
RMSD bond angle | 1.477 |
Data reduction software | DIALS (0.7.4) |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 109.390 | 109.390 | 1.970 |
High resolution limit [Å] | 1.920 | 9.010 | 1.920 |
Rmerge | 0.055 | 0.013 | 1.852 |
Rmeas | 0.066 | 0.016 | 2.264 |
Rpim | 0.036 | 0.008 | 1.282 |
Number of reflections | 38620 | 452 | 2455 |
<I/σ(I)> | 14.5 | ||
Completeness [%] | 99.7 | ||
Redundancy | 6.2 | 5.2 | 5.1 |
CC(1/2) | 0.999 | 0.999 | 0.626 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM HEPES-NaOH buffer pH 6.5 and 20% PEG 4000 |