7EQS

Crystal structure of capsid P domain of norovirus GI.3 DSV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-02
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	63.543, 100.980, 103.366
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.1871
Rwork	0.185
R-free	0.22910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p25
RMSD bond length	0.007
RMSD bond angle	1.234
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.086	0.048	0.374
Number of reflections	38522	2151	1476
<I/σ(I)>	10.6
Completeness [%]	97.4	99.7	76.2
Redundancy	6.8	6.8	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	15.5% (w/v) polyethylene glycol 3350, 0.2M Sodium tartrate dibasic dihydrate