7EPS
Partial Consensus L-threonine 3-dehydrogenase (E-change)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.108, 180.932, 141.713 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 49.400 - 2.102 |
| Rwork | 0.207 |
| R-free | 0.23480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wmx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.607 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 2.220 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.087 | 0.570 |
| Number of reflections | 878278 | 18253 |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7 | |
| CC(1/2) | 1.000 | 0.895 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20% [w/v] PEG 3350, 0.2M magnesium acetate |
