7EPR
Partial Consensus L-threonine 3-dehydrogenase (C-Change)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.019, 58.474, 138.130 |
| Unit cell angles | 90.00, 92.23, 90.00 |
Refinement procedure
| Resolution | 46.000 - 2.200 |
| Rwork | 0.208 |
| R-free | 0.25820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wmx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.476 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.074 | 0.559 |
| Number of reflections | 412432 | 8720 |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.999 | 0.847 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 275 | 20% [w/v] PEG 3350, 0.2M sodium phosphate monobasic monohydrate |
