7EP8

Crystal structure of PCNA from Neurospora crassa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2019-09-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9794
Spacegroup name	H 3
Unit cell lengths	86.290, 86.290, 91.940
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.150 - 1.950
R-factor	0.19794
Rwork	0.196
R-free	0.23499
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tup
RMSD bond length	0.004
RMSD bond angle	0.998
Data reduction software	iMOSFLM (7.3.0)
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP (11.7.02)
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.150	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.105
Rmeas	0.129
Rpim	0.075
Number of reflections	18622	1336
<I/σ(I)>	1.9
Completeness [%]	99.9	100
Redundancy	5.9	5.8
CC(1/2)	0.994	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291.15	0.2 M calcium acetate, 20% PEG 3350