7EP7

The complex structure of Gpsm2 and Whirlin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	91.885, 91.885, 177.202
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.940 - 2.600
R-factor	0.2362
Rwork	0.231
R-free	0.28320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ro2
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.940	45.940	2.690
High resolution limit [Å]	2.600	5.590	2.600
Rmerge	0.084	0.025	1.530
Rmeas	0.087	0.026	1.579
Number of reflections	14324	1596	1393
<I/σ(I)>	22.7	75.9	1.8
Completeness [%]	100.0	99.94	99.71
Redundancy	15.93	14.24	16.52
CC(1/2)	1.000	1.000	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	1.2 M Ammonium tartrate dibasic, 0.1 M Tris, pH 8.8