7EP5

Crystal structure of ZER1 bound to GKLH degron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2020-12-26
Detector	MAR CCD 165 mm
Wavelength(s)	0.979191
Spacegroup name	P 21 21 2
Unit cell lengths	69.400, 150.670, 56.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.800 - 2.020
Rwork	0.185
R-free	0.23250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ep0
RMSD bond length	0.007
RMSD bond angle	0.831
Data reduction software	XDS (0.7.4)
Data scaling software	XDS (0.7.4)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.800	2.070
High resolution limit [Å]	2.020	2.020
Rmerge	0.086	0.735
Number of reflections	39427	2746
<I/σ(I)>	18.4	2.3
Completeness [%]	99.4
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.22 M Ammonium formate and 18% (wt/vol) Polyethylene glycol 3,350