7EP1

Crystal structure of ZYG11B bound to GFLH degron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2020-11-10
Detector	MAR CCD 165 mm
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	53.090, 97.580, 121.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.843 - 1.852
Rwork	0.184
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ep0
RMSD bond length	0.007
RMSD bond angle	0.754
Data reduction software	XDS (0.7.4)
Data scaling software	XDS (0.7.4)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.170	1.900
High resolution limit [Å]	1.850	1.850
Rmerge	0.059	0.494
Number of reflections	53802	3658
<I/σ(I)>	26.96
Completeness [%]	92.7
Redundancy	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M MES pH 6.5, 14.4% (wt/vol) Polyethylene glycol 20,000