7EP0

Crystal structure of ZYG11B bound to GSTE degron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2020-11-09
Detector	MAR CCD 165 mm
Wavelength(s)	0.979214
Spacegroup name	P 43 21 2
Unit cell lengths	131.495, 131.495, 87.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.750 - 2.160
Rwork	0.190
R-free	0.22210
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.161
Data reduction software	XDS (0.7.4)
Data scaling software	XDS (0.7.4)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.980	2.270
High resolution limit [Å]	2.160	2.160
Rmerge	0.079	0.812
Number of reflections	39160	4143
<I/σ(I)>	26.7
Completeness [%]	92.4
Redundancy	15.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M MES pH 6.5, 14.4% (wt/vol) Polyethylene glycol 20,000, 0.033% (wt/vol) Anthrone, 0.033% (wt/vol) Congo Red, 0.033% (wt/vol) N-(2-Acetamido)-2-aminoethanesulfonic acid, 0.002 M HEPES sodium pH 6.8