7EO9

Crystal structure of KIF1A Motor-Neck domain with ADP-Mg-AlFx

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	41.502, 57.319, 154.826
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.680 - 2.570
R-factor	0.2259
Rwork	0.225
R-free	0.23890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vfx
RMSD bond length	0.007
RMSD bond angle	1.529
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.630
High resolution limit [Å]	2.570	2.570
Rmerge	0.081	0.517
Rpim	0.063	0.307
Number of reflections	11987	859
<I/σ(I)>	10.1	1.8
Completeness [%]	96.8	100
Redundancy	6.1	6.5
CC(1/2)	0.997	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 3350, Ammonium acetate, Bis-Tris