7ENL

MECHANISM OF ENOLASE: THE CRYSTAL STRUCTURE OF ENOLASE-MG2+-PHOSPHOGLYCERATE(SLASH) PHOSPHOENOLPYRUVATE COMPLEX AT 2.2-ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 42 21 2
Unit cell lengths	122.000, 122.000, 67.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.200
R-factor	0.169
RMSD bond length	0.015
RMSD bond angle	0.051
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	2.200 *
Rmerge	0.075 *
Total number of observations	16196 *
Number of reflections	21660 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enolase	3 (%)
2	1	drop		2 (mM)
3	1	drop	EDTA	1 (mM)
4	1	drop	dithiothreitol	1 (mM)
5	1	drop	citrate	0.05 (M)
6	1	drop	ammonium sulfate	55 (%sat)
7	1	reservoir	ammonium sulfate	55 (%sat)