7EMK

Dendrorhynchus zhejiangensis ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.96003
Spacegroup name	I 4 3 2
Unit cell lengths	151.790, 151.790, 151.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.000 - 2.300
R-factor	0.1536
Rwork	0.152
R-free	0.18830
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.476
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.153	0.069	0.787
Rmeas	0.155	0.070	0.797
Rpim	0.025	0.012	0.130
Total number of observations	513539
Number of reflections	13520	769	661
<I/σ(I)>	7.2
Completeness [%]	100.0	100	100
Redundancy	38	32.7	37.4
CC(1/2)		0.999	0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291.15	0.2 M calcium chloride dihydrate, 0.1 M HEPES sodium, pH 7.5, and 28% (v/v) polyethylene glycol 400