7EME
Putative Leptospira interrogans recombinant L-amino acid oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0723 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.756, 106.229, 139.916 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.060 - 1.780 |
| R-factor | 0.1767 |
| Rwork | 0.174 |
| R-free | 0.21950 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.643 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.610 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.118 | 0.390 |
| Rmeas | 0.124 | |
| Rpim | 0.037 | |
| Number of reflections | 74369 | 4197 |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 99.1 | 99.8 |
| Redundancy | 11.3 | |
| CC(1/2) | 0.978 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 298 | 0.1 M Potassium thiocyanate, 30% w/v Polyethylene glycol monomethyl ether 2000 |
