7EL3
Crystal structure of HpaR-DNA complex from Acinetobacter baumannii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2020-07-04 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.423, 55.150, 57.072 |
| Unit cell angles | 81.14, 66.79, 67.97 |
Refinement procedure
| Resolution | 27.610 - 1.700 |
| R-factor | 0.1657 |
| Rwork | 0.164 |
| R-free | 0.19260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7el2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.236 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.610 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.022 | |
| Rmeas | 0.028 | |
| Rpim | 0.017 | |
| Number of reflections | 52505 | 2711 |
| <I/σ(I)> | 19.1 | 5.2 |
| Completeness [%] | 96.9 | |
| Redundancy | 2.4 | |
| CC(1/2) | 1.000 | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 100 mM sodium HEPES; MOPS, pH 7.5, 20 mM DL-glutamic acid, 20 mM DL-alanine, 20 mM glycine, 20 mM DL-lysine, 20 mM DL-serine, 20 % (v/v) glycerol and 10 % (v/v) PEG 4000 |
