7EL1

Structure of a protein from bacteria

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-BM
Synchrotron site	APS
Beamline	17-BM
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	330.585, 105.222, 68.551
Unit cell angles	90.00, 92.71, 90.00

Refinement procedure

Resolution	50.000 - 2.220
Rwork	0.213
R-free	0.24230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5czz
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (v1.11)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.470	2.340
High resolution limit [Å]	2.220	2.220
Rmerge	0.052	0.714
Number of reflections	116344	16935
<I/σ(I)>	17
Completeness [%]	99.9
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.0M sodium citrate dihydrate, 0.3M imidazol-Hcl (pH 10.0), 0.02M sodium malonate (pH 7.0)