7EKJ

Crystals structure of classical swine fever virus NS5B (residues 91-694)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 43 21 2
Unit cell lengths	82.064, 82.064, 228.059
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.490 - 3.060
R-factor	0.2687
Rwork	0.268
R-free	0.28340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yf6
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (v1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.000	60.000	3.170
High resolution limit [Å]	3.060	6.590	3.060
Rmerge	0.084	0.044	0.536
Rmeas	0.088	0.046	0.567
Rpim	0.028	0.014	0.179
Total number of observations	133805
Number of reflections	14282	1693	1104
<I/σ(I)>	6.6
Completeness [%]	92.6	97.7	74.5
Redundancy	9.4	8.7	8.9
CC(1/2)		0.999	0.937

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	289	Ammonium sulfate