7EKF

Structure of SARS-CoV-2 Alpha variant spike receptor-binding domain complexed with human ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	104.530, 104.530, 230.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.860 - 2.850
R-factor	0.2089
Rwork	0.208
R-free	0.22380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lzg
RMSD bond length	0.005
RMSD bond angle	0.695
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.120	2.920
High resolution limit [Å]	2.850	2.850
Number of reflections	30710	30617
<I/σ(I)>	16.5
Completeness [%]	99.9
Redundancy	21.7
CC(1/2)	0.999	0.684

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M MES, pH 6.5, 10% w/v PEG 5000 MME, 12% v/v1-Propanol