7EKE

Structure of SARS-CoV-2 spike receptor-binding domain F486L mutation complexed with human ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	104.316, 104.316, 228.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.500 - 2.700
R-factor	0.2133
Rwork	0.212
R-free	0.24070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lzg
RMSD bond length	0.005
RMSD bond angle	0.691
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	35606	34993
<I/σ(I)>	20.354
Completeness [%]	100.0
Redundancy	19
CC(1/2)	0.996	0.771

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M MES, pH 6.5, 10% w/v PEG 5000 MME, 12% v/v1-Propanol