7EK9

Crystal structure of apo streptavidin at cryogenic temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	46.373, 85.946, 58.188
Unit cell angles	90.00, 98.73, 90.00

Refinement procedure

Resolution	17.896 - 1.100
R-factor	0.1602
Rwork	0.159
R-free	0.18710
Structure solution method	FOURIER SYNTHESIS
Data reduction software	XDS
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	18.000	18.000	1.140
High resolution limit [Å]	1.100	2.370	1.100
Rmerge	0.058	0.045	0.354
Rmeas	0.074	0.058	0.464
Rpim	0.045	0.036	0.296
Total number of observations	316645
Number of reflections	147496	14578	8700
<I/σ(I)>	12.9
Completeness [%]	80.7	78.9	47.8
Redundancy	2.1	2.1	1.7
CC(1/2)		0.993	0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6	294	Pact PremierTM 100 mM MMT buffer, PEG 1500