7EK6
Structure of viral peptides IPB19/N52
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 296 |
| Detector technology | PIXEL |
| Collection date | 2020-11-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 38.233, 38.233, 317.329 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.932 - 1.243 |
| R-factor | 0.1737 |
| Rwork | 0.173 |
| R-free | 0.18540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lxt |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.932 | 3.710 |
| High resolution limit [Å] | 1.240 | 1.240 |
| Rmerge | 0.058 | 1.220 |
| Number of reflections | 25377 | 3470 |
| <I/σ(I)> | 35 | 2.1 |
| Completeness [%] | 97.1 | 84 |
| Redundancy | 31.47 | 14.1 |
| CC(1/2) | 0.950 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 296 | 1.26M Sodium phosphate monobasic monohydrate, 0.14M Potassium phosphate dibasic, pH 5.6 |
