7EK0

Complex Structure of antibody BD-503 and RBD-N501Y of COVID-19

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	81.426, 149.665, 146.433
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.540 - 2.700
R-factor	0.2182
Rwork	0.214
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bwj
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.540	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.232
Rmeas	0.242
Rpim	0.067	0.341
Number of reflections	23686	1595
<I/σ(I)>	16.64	1.84
Completeness [%]	94.7
Redundancy	13.1	13.6
CC(1/2)	0.991	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Sodium citrate, pH 5.5, 15% (w/v) PEG 6000