7EJG

Crystal structure of PBP domain of RMCA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-07-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.03320
Spacegroup name	P 61
Unit cell lengths	109.330, 109.330, 41.533
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.110 - 1.680
R-factor	0.199
Rwork	0.197
R-free	0.22280
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.105
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.16_3549)
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.110	1.740
High resolution limit [Å]	1.680	1.680
Number of reflections	32306	3200
<I/σ(I)>	3.07
Completeness [%]	99.2
Redundancy	10.4
CC(1/2)	1.000	0.916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	2%PEG550, 1.8M Ammonium sulfate, 0.1M BIS-TRIS hydrochloride