7EJB

Crystal structure of juvenile hormone acid methyltransferase JHAMT mutant Q15E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 32 1 2
Unit cell lengths	100.820, 100.820, 70.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.820 - 2.850
R-factor	0.2442
Rwork	0.243
R-free	0.27620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ebs
RMSD bond length	0.002
RMSD bond angle	1.212
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.820	2.920
High resolution limit [Å]	2.850	2.850
Rmerge	0.155	1.487
Rmeas	0.160
Number of reflections	9668	711
<I/σ(I)>	15.7	2.1
Completeness [%]	99.6
Redundancy	19.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	289	1.1M Ammonium tartrate dibasic, 0.1M Sodium acetate trihydrate pH 4.6