7EIW
Human histidine decarboxylase mutant Y334F reacted with histidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.902, 57.654, 117.647 |
| Unit cell angles | 90.00, 106.30, 90.00 |
Refinement procedure
| Resolution | 33.223 - 2.100 |
| Rwork | 0.212 |
| R-free | 0.25210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e1o |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.319 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.069 | 0.431 |
| Number of reflections | 52603 | 5121 |
| <I/σ(I)> | 20.6 | |
| Completeness [%] | 99.6 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 0.1 M Tris-HCl (pH 8.5), 28% (w/v) PEG 3350, 0.2 M lithium sulfate |
