7EIU

Crystal structure of Mei2 RRM3 in complex with 8mer meiRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	66.227, 162.119, 96.657
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.114 - 2.349
R-factor	0.184
Rwork	0.182
R-free	0.22480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7eio
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.114	2.390
High resolution limit [Å]	2.349	2.349
Rmerge	0.119	0.810
Number of reflections	21980	1059
<I/σ(I)>	18.15	2.91
Completeness [%]	99.9
Redundancy	7.3
CC(1/2)	0.994	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Ammoniun Sulfate, 20% PEG3350