7EII

Ancestral L-Lys oxidase K387A variant (L-Lys binding form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	85.829, 80.086, 87.986
Unit cell angles	90.00, 90.42, 90.00

Refinement procedure

Resolution	42.900 - 2.400
Rwork	0.183
R-free	0.23080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7eih
RMSD bond length	0.007
RMSD bond angle	1.460
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.900	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.087	0.421
Number of reflections	46762	6740
<I/σ(I)>	16.2
Completeness [%]	99.8
Redundancy	6.8
CC(1/2)	0.998	0.926

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25%(w/v) PEG3350, 0.1 M Tris-HCl (pH 8.5)