7EHP
Chitin oligosaccharide binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-03 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 195.965, 49.798, 77.865 |
| Unit cell angles | 90.00, 107.12, 90.00 |
Refinement procedure
| Resolution | 38.930 - 2.010 |
| R-factor | 0.1777 |
| Rwork | 0.175 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7eho |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.214 | 1.098 |
| Rpim | 0.099 | 0.511 |
| Number of reflections | 47871 | 2402 |
| <I/σ(I)> | 12.7 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.8 | 4.6 |
| CC(1/2) | 0.642 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | PEG4000, sodium acetate, Tris |
