7EHF
Crystal structure of the aminoglycoside resistance methyltransferase NpmB1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.738, 65.798, 65.806 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.840 - 1.500 |
| R-factor | 0.1671 |
| Rwork | 0.166 |
| R-free | 0.19380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rwz |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.073 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 1.343 |
| Number of reflections | 30430 | 4807 |
| <I/σ(I)> | 25.86 | 2.85 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 26.2 | 26.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% PEG3350, 100 mM HEPES pH 7.0, 5% ethyleneglycol |
