7EGY
Recombinant head-to-tail dimeric E2 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 63 |
| Unit cell lengths | 177.170, 177.170, 59.171 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.555 - 4.201 |
| R-factor | 0.3878 |
| Rwork | 0.381 |
| R-free | 0.44410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yq2 |
| Data reduction software | xia2 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.530 |
| High resolution limit [Å] | 4.200 | 4.470 |
| Rmerge | 0.115 | |
| Rmeas | 0.119 | |
| Rpim | 0.028 | |
| Number of reflections | 130097 | 6628 |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 10.1 | |
| CC(1/2) | 0.997 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M Magnesium formate and 15% (w/v) PEG 3350 |
